The date of birth of the Department is 15 September 1946 when the entomologist, a specialist in the field of plant protection, candidate of agricultural sciences, associate professor S. F. Segeda joined the Faculty of Biology and estabished the Department of Inverterbrate Zoology. He was the first head of the Department and its leader. Stepan Fedorovych started and determined the entomological educational and scientific direction of the newly formed subdivision of the Faculty of Biology. In May 1956 the Department of Invertebrate Zoology was the most numerous at the Faculty of Biology. The full-time staff included: Head of the Department, Associate Professor К. К. Fasulati, Senior Lecturers Т. А. Tveritina, H. M. Roshko, Assistants І. М. Lykovych, А. А. Hirits, Senior laboratory assistant І. І. Bokotey, Senior assistant Е. N. Hashak, assistant V. H. Sofin. In autumn 1956 the Department of Invertebrate Zoology was joined to the Department of Vertebrate Zoology. The newly formed Department of Zoology existed in this form for ten years. However, the educational and scientific direction of zoology of invertebrate at the Faculty of Biology was not eliminated, did not lose its face and potential. Despite the formal unity during all these years the Department of Zoology majored in zoology of invertebrate. In September 1966 the legal successor the Department of Invertebrate Zoology separated from the Department of Zoology in its old superior quality, but with a new, more appropriate name – Department of Entomology. Its Head was Doctor of Biological Sciences, Professor V. F. Paliy who came to Uzhhorod from the city of Frunze, where he was Head of the Laboratory of Entomology at the Institute of Biology of Kirghiz Academy of Sciences.

The Department of Entomology as one of the first departments at the Faculty of Biology of Uzhhorod university is well known for its traditions of classical academism in its professional activity. These positive traditions were formed by the following Heads of Department: S. F.Segeda, V. F. Paliy, К. К. Fasulati, J. М. Pohoryliak, H. M. Roshko, І. І. Bokotey, І. Ya.Ponin. The performers of the educational and scientific tasks of the Department were the following lecturers: І. І. Zabolotskyi, Т. А. Tveritina, H. М. Roshko, А. А. Hirits, І. М. Lykovych, А. А. Zubenko, Z. L. Anikina, V. О. Dobey, М. F. Mateleshko, Zh.H. Melika, V. Yu. Krochko, І. V. Zagorodniuk.

In 2006 the Department was renamed into the Department of Entomology and Biodiversity Conservation. Today, the Department employs Associate Professors: Vladyslav Mirutenko (Head of the Department), Volodymyr Roshko, Oleksandr Mateleshko, Myroslava Demchynska, Lyudmyla Symochko, Maksym Chumak, Pavlo Lovas and laboratory assistants: Mykhailo Matkovskyi, Tetiana Pidhorodska, Volodymyr Lovas, Olha Lovas. The friendly pedagogical staff of the Department is famous for the proficiency of its staff both in the theoretical and practical spheres of entomology, general biology, biodiversity monitoring, general and applied ecology, parasitology, phytopathology.

The Department staff regularly participate in international research programs, fellowships and scholarships at domestic and foreign scientific institutions, and carry out research projects at partner institutions.

In recent years, the Department's staff have been actively involved in international projects. These include Partnership for Genomic Research in Ukraine and Romania, Environment for the Future by Scientific Education, Carpathian Youth for Ecosmart Education, and Environment for the Future by Scientific Education – Work in Nature.

Since 2001, an annual scientific entomological conference, “Uzhhorod Entomological Readings,” has been held, which is highly regarded by leading entomologists in Ukraine and has already gained international status. “Uzhhorod Entomological Readings” is a traditional forum of entomologists of the Carpathian-Ukrainian region. This is an annual scientific conference on general and applied entomology and nature conservation in the Carpathians, organized by the Department of Entomology and Biodiversity Conservation of the Uzhhorod National University and the Transcarpathian Branch of the Ukrainian Entomological Society.