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05.09.2020
2023

Uzhhorod National University started performing ELISA testing for COVID-19

Uzhhorod National University started performing ELISA testing for COVID-19

There is a Research and Training Center for Molecular Microbiology and Immunology of Mucous Membranes actively functioning at UzhNU (The head of the Center,  Doctor of Biology, prof. Nadiya Boyko), whose staff regularly participates in domestic and international projects (including those funded by Horizon 2020), cooperates with many institutions, including medical and diagnostic centers of the region (Prevention, Astra-DIA, One LAB).

Even before the current pandemic, understanding the priorities of modern science development, the administration of UzhNU purchased state-of-the-art equipment needed for microbiological, genetic and immunological research:, including an amplifier for performing PCR in real time, a homogenizer for DNA extraction, laminar flow cabinets (2nd safety class), thermoshakers and a spectrophotometer for quantitative assessment of extracted DNA, an ELISA reader with automatic sample preparation system, etc.

This equipment allows to take various clinical measurements, including the determination of the presence of IgM / IgG antibodies to the coronavirus SARS-CoV-2 in serum by enzyme-linked immunosorbent assay (ELISA). Enzyme-linked immunosorbent assay shows whether a person has an acute form of the disease (IgM) has already recovered from it or had it without any symptoms (IgG).

 Thus, due to the difficult epidemiological situation in the country and the region, Uzhhorod National University purchased sufficient amount of ELISA tests to test its employees, and obtain statistical data on the strength and duration of immunity of the population of Uzhhorod in the future. What’s Important, a person doesn’t need to have a physician’s referral for ELISA testing. Diagnostics is performed by the staff of the Center for Molecular Microbiology and Immunology of Mucous Membranes in cooperation with the Medical Center “Prevention” (Chief Physician Viktor Petrov).

Enzyme-linked immunosorbent assay (ELISA) is a type of assay that detects an "antigen-antibody" complex that is produced when a body has been infected with a virus. This complex is formed due to the immunological reaction of the antigen with the corresponding antibody. The ELISA method determines two types of immunoglobulins: M and G. If M is detected, it indicates an acute disease process, if G is detected, it means that a person was ill or infected with the virus and developed antibodies to this disease. The use of ELISA tests for the detection of COVID-19 antibodies is regulated by the order of the Ministry of Health dated as of 20.05.2020 №1227 "On Approval of Changes to the Standards of Medical Care "Coronavirus disease " (COVID-19).

A positive ELISA test result is an additional basis for PCR testing. At present, PCR remains the only type of testing that allows to establish a diagnosis "COVID-19". They are planning to launch the PCR testing at UzhNU in the near future.

The purchase of equipment, availability of highly qualified personnel and laboratories will allow UzhNU to obtain the necessary permits and certification documents for diagnosing diseases, including by means of PCR testing, and for conducting scientific clinical trials in collaboration with other clinical teams.

 

 

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